Dianthus uHTS
Screen faster.
Discover more.
Ultra high-throughput speed. Biophysical confidence.
Ultra high-throughput speed. Biophysical confidence.
Screen across diverse targets and binding modes.
A million compounds screened in five days.
Walk-away automation that fits your workflow.
Take your analysis further with structured, AI-ready data exports.
From primary screening to hit validation, Dianthus uHTS handles the full spectrum of early-stage drug discovery workflows with biophysical precision.
technology tag
Prometheus™ Panta is powered by our unique technologie
DLS enables you to monitor the colloidal stability of your sample. This information helps you optimize your sample by making changes to the sequence or buffer environment, and measuring how those changes impact colloidal stability.
Hit ID and hit-to-lead for HTS compound libraries.
Screen hundreds of thousands to millions of compounds with direct target engagement data. Identify hits with confidence, knowing you’re measuring true binding, not proxy signals. Achieve robust Z′-factors that validate your screening campaign and give you hits worth pursuing.
One readout.
Multiple insights.
Dianthus uHTS uses Spectral Shift technology, engineered for 1536-well throughput.
Multiple insights.
Spectral Shift
Dianthus uHTS relies on Spectral Shift technology. The process is simple: label one binding partner with a fluorophore, then mix a fixed amount of it with a dilution series of the ligand. After excitation at 590 nm of the mix, the binding events are detected as shifts of spectra towards either blue or red. The detection of spectral shifts is achieved through dual-wavelength detection at 650 nm and 670 nm in an isothermal environment. This makes Dianthus uHTS precise enough to detect very subtle shifts, even when taking extremely rapid measurements. Then the Kd is calculated by plotting the ratios of the fluorescence intensities from the two wavelengths against the ligand concentration.
“NanoTemper helps us turn challenging biophysical tasks into routine workflows. Their intuitive solutions give us reliable data faster, so our teams can focus on advancing drug candidates.”
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More about Dianthus uHTS.
Complete your setup.
Get reliable stability data.
Without worrying about sample prep.
Dianthus uHTS 1536-well plates are manufactured using the same stringent protocols used for diagnostic-grade materials to ensure the best results. Reagent kits offer flexible labeling so you can spend less time optimizing assays and more time collecting data.
Go from experiment to insight. Without the complexity.
Getting started with affinity experiments is simple with Dianthus uHTS. Control software is designed is designed to plug in your existing scheduler using OPEN and FAIR APIs. Human and machine readable .json file formats also allow for rapid integration of instrument data into your analysis pipeline; whether it’s third party software or open source solutions.
Scale up screening.
Without the hands-on time.
For high-throughput screening screening, Dianthus uHTS is fully integratable with your existing automation solution. Use OPEN and FAIR APIs to manage your instrument and export data.
Maximize instrument performance.
Without the Downtime.
NanoTemper instrumentation is built to the highest standards of quality and reliability; however, it’s important to keep your instruments in good working order to ensure accurate, high-quality data output. Don’t let unexpected delays slow you down or interrupt your lab workflows. Service offerings include: relocation services, operational qualification procedures, and full coverage repair and validations.
FAQs
Dianthus uHTS
Dianthus uHTS provides direct target engagement data without requiring knowledge of the binding site or functional readout. This makes it ideal for targets where biochemical or FRET-based assays are difficult to establish. You get biophysical confirmation of binding, not just activity.
Measure 1536 data points fully automated in less than 10 minutes. You can screen 1,000,000 compounds with robust Z' scores (>0.5, typically >0.8) in 5 days.
As little as 7 μL per well for 1536-well plates, or 9 μL per well for 384-well plates. Target concentrations can be as low as 250 pM, minimizing sample consumption even at HTS scale.
Yes. Dianthus uHTS integrates into existing automation systems via gRPC framework for walk-away operation and hands-free workflow processing. It can also operate as a standalone device via control software.
From millimolar to sub-nanomolar (down to 250 pM). This wide dynamic range lets you screen fragments, small molecules, and tight binders without changing your assay setup.