Andromeda X
Crude lysates.
Early decisions.
Screen and optimize expression conditions for your toughest recombinant proteins. Get protein stability data from crude lysates with the sensitivity to detect low-yield targets. Proceed with confidence to the purification of folded, functioning proteins.
More insight. Fewer setbacks. Better proteins.
More insight. Fewer setbacks. Better proteins.
Get expression and stability data fast.
Work directly in crude lysates from many different organisms, including E. coli, HEK293, and Sf9.
Know more before you commit.
Confirm your protein is functional with in-lysate thermal shift assays.
Instrument, software, and consumables designed to work in harmony.
Deeper insight into your recombinant proteins, earlier in your workflow.
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Prometheus™ Panta is powered by our unique technologie
DLS enables you to monitor the colloidal stability of your sample. This information helps you optimize your sample by making changes to the sequence or buffer environment, and measuring how those changes impact colloidal stability.
Your challenging proteins, measurable early.
Get expression level and protein stability data at every stage of your protein production workflow, from small-scale screening to pre-purification quality checks. Work directly in crude lysates to make informed decisions before committing to scale-up or purification.
One readout.
Multiple insights.
One technology. Expression and stability in one assay. Free from purification.
Multiple insights.
Spectral Shift
Andromeda X uses highly sensitive Spectral Shift optics to selectively detect your protein of interest in crude lysate. A site-specific fluorescent label is added to the lysate containing the recombinant protein, and as a temperature gradient is introduced, the optics monitor changes in the fluorophore’s environment to detect protein unfolding.
The ratio of emitted fluorescence at 650 nm and 670 nm is used to follow protein unfolding and plot the inflection point (Ti). Compare peak heights of different expression conditions to select those that result in high expression of folded protein. Use shifts in the Ti to find conditions that stabilize your protein or to confirm ligand binding.
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More about Andromeda.
Complete your setup.
Get reliable stability data.
Without worrying about sample prep.
All you need for Andromeda X experiments are capillaries and labeling kits. Andromeda X capillaries are manufactured using the same stringent protocols used for diagnostic-grade materials to ensure the best results. NanoTemper dyes are optimized for Spectral Shift to achieve the highest possible data quality. Our pre-packaged kits come with protocols optimized to ensure out-of-the-box success.
Software that's easy to use and easy to learn.
Getting started with is simple with Andromeda software. Control software is designed for easy, customizable set-up of experiments and allows you to view data acquisition in real time. Powerful analysis software enables you to sort, highlight, and rank your conditions. All software has a clean interface and intuitive user experience, so you’re ready to get started as soon as your Andromeda fires up.
Maximize instrument performance.
Without the Downtime .
NanoTemper instrumentation is built to the highest standards of quality and reliability; however, it’s important to keep your instruments in good working order to ensure accurate, high-quality data output. Don’t let unexpected delays slow you down or interrupt your lab workflows. Service offerings include: relocation services, operational qualification procedures, and full coverage repair and validations.
FAQs
Andromeda X
Andromeda X uses only 10 μL of lysate per measurement. Highly sensitive optics detect your protein of interest even at low expression levels, giving you Coomassie-level sensitivity with additional functional information in just 15 minutes.
At the maximum heating rate of 7.0 °C/min, a full experiment with 48 samples takes less than 11 minutes. You can find the Ti values for your recombinant proteins in about 15 minutes, including labeling and measurement.
Andromeda X works with lysates from bacterial (E. coli), mammalian (HEK293), and insect (Sf9) expression systems, as well as with cell culture supernatants for secreted proteins.
No. Andromeda X works directly in crude lysates, so you can evaluate expression levels and thermal stability before committing to any purification scheme. This lets you eliminate unpromising constructs early and save time and resources.
NanoTemper offers site-specific, one-step labeling kits for His-tagged (Tris-NTA), SNAP-tagged, biotinylated, and Human Fc-tagged proteins. Covalent labeling kits are also available for pre-checking binding conditions or retained protein functionality ahead of other screening methods.