One experiment.
Multiple insights.

• Monoclonal antibodies (IgGs, fragments, bispecifics)
• Fusion proteins and multi-domain constructs
• Therapeutic enzymes
• Membrane Proteins
• Peptide based therapeutics
• Gene therapy vectors (AAV capsids)
• Protein-based vaccines and VLPs
• Novel scaffolds (nanobodies, DARPins, affibodies)
• ADCs and PEGylated proteins

• Combinatorial Approach:
  Assess the developability of your biologics during thermal unfolding by monitoring intrinsic fluorescence, DLS, and backreflection in a single experiment.
• Thermal shift assays:
  Use thermal unfolding measurements to screen for small molecule drug candidate compounds that increase the stability of your target protein, indicating an interaction.
• Self-association determination:
  With size analysis measurement type, determine if a protein is more likely to self-associate, and therefore become viscous or aggregated.
• Chemical stability assessment:
  Do chemical unfolding experiments to find the free energy of unfolding of your proteins.
• Accelerated stability studies:
  Extrapolate the long-term stability of your proteins with high-temperature isothermal scan measurements.
• Thermal Stress studies:
  Precisely assess the impact of thermal stress on your biologics and model process conditions with customizable thermal profiles using temperature control.

• Purity
• Polydispersity index (PDI)
  PDI represents distribution of size populations
  Thermal Unfolding
• T_m (for 330 nm, 350 nm, and ratio)
  Melting temperature, or point at which 50% is unfolded
• T_onset (for ratio)
  Temperature at which thermal unfolding begins, measured from fluorescence ratio
• Ea(*parameter derived from data)
  Activation energy of unfolding (*parameter derived from data)
  Reversibility of unfolding
  Point at which irreversibility happens when unfolding, thereby making aggregation more likely
  Sizing Information
• r_H
  Hydrodynamic radius shows size of particle in solvated state
• Molecular weight
  Average molecular weight of all particles in solution
• T_size
  Temperature at which average particle size begins to increase – measured from from growth of r_H in cumulants fit
• Average scattering intensity
  Aggregation and Self-Association
• T_turbidity
  Onset temperature of turbidity, or large aggregates >12.5 nm radius
• T_scattering
  Temperature at which scattering intensity begins to change, indicating increased size and aggregation
• B₂₂
  Second virial coefficient used to extrapolate self-association behavior at higher concentrations
• k_d
  Diffusion interaction identifies onset of unfolding and impact on colloidal stability
  Chemical Denaturation
  C_m or C₅₀
  The concentration of a denaturant that causes 50% of proteins to unfold
• ΔG
  The Gibbs free energy of protein unfolding, a thermodynamic measure of the likelihood a folding event may occur

Yes. Prometheus Panta supports automated sample loading, enabling high-throughput workflows without sacrificing data quality. Its standardized protocols make it straightforward to integrate into existing automation pipelines, reducing hands-on time and minimizing variability across runs.

The Prometheus Panta C is purpose-built software package for regulated environments, supporting use settings where 21 CFR Part 11 compliance and data integrity are critical. It is designed to meet the demands of GMP-adjacent workflows, with features that support method validation, audit trails, and the rigorous documentation standards required in biopharmaceutical production.